Intact Proinsulin ELISA

The Invitron Intact Proinsulin Assay is an immunometric assay for the quantitative measurement of intact proinsulin in human samples.

Key features include:

  • Easy to use – very simple assay protocol
  • High sensitivity detection system
  • High specificity for ‘intact’ proinsulin
  • Highly specific marker for insulin resistance in type 2 diabetes
  • Calibrated against WHO International Standard (IRP 84/611)



96 well plate


plasma (recommended)

or serum

Sample volume


Working range

0 – 100 pmol/l


0.3 pmol/l


Proinsulin 100%

Insulin 0%

C-Peptide 0%

32-33 split proinsulin: 5.6%

Des 31-32 split proins: 1.4%

65-66 split proinsulin: 37%*

Des 64-65 split proins: 63%*

*not present at detectable

levels in human samples


1 x 2 hours, 1 x 1 hour

1 x 30 min

Product code



Add 50 μl sample buffer and 50 μl sample to each well

Incubate for 2 hours at room temp

Wash 3 times and add labelled antibody

Incubate for 1 hour at room temp

Wash 3 times and add enzyme

Incubate for 30 min at room temp

Wash 3 times and add substrate

Incubate for 20 min at room temp

Add stop solution and read at 450nm