RBP4 ELISA

The Invitron RBP4 ELISA is an immunometric assay for the quantitative measurement of retinol binding protein 4 (free RBP4 and complex bound RBP4) in human samples.

Key features of the assay include:

  • A new marker used in studies of insulin resistance

  • Indicator of chronic liver disease

  • Useful for early detection of proteinuria

Characteristics

Format

96 well plate

Specimen

serum, plasma, urine

Sample volume

20μl

Working range

0 – 30 μg/l

Incubations

2 x 1 hours, 1 x 15 min

Product code

IV3-101E

Characteristics

Add 100 μl of diluted sample to each well

Incubate for 1 hour at room temp

Wash 5 times and add conjugate

Incubate for 1 hour at room temp

Wash 5 times and add substrate

Incubate for 15 min at room temp

Add stop solution and read at 450nm