MLT Intact Proinsulin Luminescence

The Invitron Intact Proinsulin Assay is an immunometric assay for the quantitative measurement of intact proinsulin in human samples.

Key features include:

  • Easy to use – very simple assay protocol

  • High sensitivity detection system

  • High specificity for ‘intact’ proinsulin

  • Highly specific marker for insulin resistance in type 2 diabetes

  • Calibrated against WHO International Standard (IRP 84/611)

Characteristics

Format

96 well plate

Specimen

plasma (recommended)

or serum

Sample volume

50μl

Working range

0 – 100 pmol/l

Sensitivity

0.02 pmol/l

Specificity

Proinsulin 100%

Insulin 0%

C-Peptide 0%

32-33 split proinsulin: 5.6%

Des 31-32 split proins: 1.4%

65-66 split proinsulin: 37%*

Des 64-65 split proins: 63%*

*not present at detectable

levels in human samples

Incubations

1 x 2 hours, 1 x 1 hour

Product code

IV2-002

Protocol

Add 50 μl sample buffer and 50 μl sample to each well

Incubate for 2 hours at 37°C

Wash 3 times and add 100 μl labelled antibody solution

Incubate for 1 hour at 37°C

Wash 3 times

Read in luminometer