hsCRP ELISA

The Invitron hsCRP ELISA is an immunometric assay for the quantitative measurement of C-reactive protein in human samples.

Key features of the assay include:

  • A predictive marker for cardiovascular disease
  • Useful for monitoring CVD therapies

Characteristics

Format

96 well plate

Specimen

serum or plasma

Sample volume

10μl

Working range

0 – 150 ng/ml

Sensitivity

1.9 ng/ml

Incubations

2 x 1 hours, 1 x 15 min

Product code

IV3-105E

Protocol

Add 100 μl of diluted sample to each well

Incubate for 1 hour at room temp

Wash 5 times and add conjugate

Incubate for 1 hour at room temp

Wash 5 times and add substrate

Incubate for 15 min at room temp

Add stop solution and read at 450nm