Adiponectin ELISA

The Invitron Adiponectin ELISA is an immunometric assay for the quantitative measurement of adiponectin in human samples.

Key features of the assay include:

  • Provides valuable information in studies of metabolic syndrome
  • A marker of T2 diabetes, coronary artery disease, atherosclerosis

Characteristics

Format

96 well plate

Specimen

serum or plasma

Sample volume

25μl

Working range

0 – 90ng/ml

Incubations

2 x 1 hour, 1 x 15 min

Product code

IV3-102E

Protocol

Add 100μl of diluted sample to each well

Incubate for 1 hour at room temp

Wash 5 times and add conjugate

Incubate for 1 hour at room temp

Wash 5 times and add substrate

Incubate for 15 min at room temp

Add stop solution and read at 450nm