C-Peptide ELISA

The Invitron C-Peptide ELISA is an immunometric assay for the quantitative measurement of C-Peptide in human samples.

Key features include:

  • Easy to use – very simple assay protocol
  • High specificity for C-Peptide
  • Calibrated against WHO International Standard (IRP 84/510)

Characteristics

Format

96 well plate

Specimen

plasma (recommended)

or serum

Sample volume

25μl

Working range

0 – 100 pmol/l

Sensitivity

10 pmol/l

Specificity

C-Peptide 100%

Insulin: 0%

Proinsulin: 2%

Incubations

1 x 2 hours, 1 x 15 min

Product code

IV2-104E

Protocol

Add 100 μl enzyme conjugate and 25 μl sample to each well

Incubate for 2 hours at room temp

Wash 3 times and add substrate

Incubate for 15 min at room temp

Add stop solution and read at 450nm