Glargine ELISA

The Invitron Glargine ELISA is an immunometric assay for the quantitative measurement of Glargine (Lantus®) in human samples.

Key features include:

  • Easy to use – very simple assay protocol
  • High sensitivity detection system
  • High specificity for Glargine

Characteristics

Format

96 well plate

Specimen

plasma

Sample volume

25μl

Working range

0 – 240mU/l

Sensitivity

0.8mU/l

Specificity

Glargine 100%

Insulin 7.5%

Detemir 0%

Lispro 0%

Aspart 0%

Glulisine 0%

Incubations

2 x 2hrs, 1 x 15min

Product code

IV2-113E

.

Protocol

Add 100 μl sample buffer and 25 μl sample to each well

Incubate 2 hours at 4°C

Wash 3 times and add 100 μl conjugate

Incubate for 2 hours at 4°C

Wash 3 times and add substrate

Incubate for 15 min at room temp

Add stop solution and read at 450nm