Chromgranin A ELISA

The Invitron CgA ELISA is an immunometric assay for the quantitative measurement of Chromogranin A in human samples.

Key features include:

  • High specificity assay – employing 2 monoclonal antibodies
  • Simple assay protocol – predilution of samples is not necessary within the working range of this assay

Characteristics

Format

96 well plate

Specimen

plasma

Sample volume

25μl

Working range

0 – 800 ng/ml

Sensitivity

ng/ml

Incubations

1 x 2 hours, 1 x 1 hour,

1 x 30 min

Product code

IV5-101E

Protocol

Add 100 μl sample buffer and 25 μl sample to each well

Incubate for 2 hours

Wash 3 times and add HRP conjugate

Incubate for 1 hour

Wash 3 times and add substrate

Incubate for 30 min

Add stop solution and read at 450nm